Extensive site-directed mutagenesis reveals interconnected functional units in the alkaline phosphatase active site
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چکیده
منابع مشابه
Extensive site-directed mutagenesis reveals interconnected functional units in the alkaline phosphatase active site
Enzymes enable life by accelerating reaction rates to biological timescales. Conventional studies have focused on identifying the residues that have a direct involvement in an enzymatic reaction, but these so-called 'catalytic residues' are embedded in extensive interaction networks. Although fundamental to our understanding of enzyme function, evolution, and engineering, the properties of thes...
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ژورنال
عنوان ژورنال: eLife
سال: 2015
ISSN: 2050-084X
DOI: 10.7554/elife.06181